Fig. 2
Inflammatory Markers in Left Ventricular Biopsies from Patients with HFpEF, with (+ DM) and without Concomitant Diabetes (−DM). A Interleukin-6 (IL-6) levels. B IL-6 expression over GAPDH. C Immunoflourescence staining of IL-6. DAPI staining (blue) and WGA (anti-wheat agglutinine 555 conjugate, red) staining are used for nucleic acids and membranes. D Fpassive at sarcomere length 1.8–2.4 µm in the presence or absence of IL-6 inhibitor. Curves are second-order polynomial fits to the means (± SEM; n = 30–36/5 cardiomyocytes/heart per group), * P < 0.05 HFpEF -DM vs. HFpEF + DM, ‡P < 0.05 HFpEF -DM vs. HFpEF -DM after IL-6inh, † P < 0.05 HFpEF + DM vs. HFpEF + DM after IL-6inh treatment by one-way ANOVA. P-values were corrected for multiple comparisons by the Tukey method. E IL-6 receptor (IL6-R) expression over GAPDH. F Immunoflourescence staining of IL6-R. G Immunoflourescence staining of myeloperoxidase (MPO). H Immunoflourescence staining of neutrophil elastase (NE). Panels A + B + E. Data are represented as box and whisker plots (median, 25th to 75th percentiles, minimum, and maximum (n = 6–10 samples/group). P-values are derived from an unpaired t-test